Abstract: In order to explore the optimum growth conditions of Sanguinolaria diphos juveniles,response surface methodology was used to study the interactions of temperature(24℃,27℃,30℃),salinity(20,25,30),pH(7.3±0.03,8.3±0.03,9.3±0.03)on Sanguinolaria diphos juveniles daily growth rate.The results showed:1)The effect of temperature on the growth rate was the most significant(P<0.01),and the order of three ecological factors effects was temperature(A)>salinity(B)>pH(C).2)By means of Design-Expert 8.0.6 software,the optimum growth environment conditions of Sanguinolaria diphos juveniles were as follows:temperature 26.43℃,salinity 25 and pH 8.3,and theoretical optimal growth rate was 2.90%.After experimental verification,the real growth rate was 2.76% under the theoretical condition,suggesting RSM was reliable.The research results provided a theoretical references for Sanguinolaria diphos artificial aquaculture in the pond.