Abstract: The quality of seawater is one of the most important environmental factors affecting the reproduction,development,metabolism and growth of Pacific white shrimp(Litopenaeus vannamei).Therefore,knowing the impacts of different types of seawater(natural seawater and artificial seawater)on white shrimp aquaculture will provide important information for the aquaculture industry.In the present study,rearing experiment and qPCR were conducted to investigate the impacts of two types of seawater on the growth performance and expression of metabolic genes of Pacific white shrimp.The results obtained showed that P.aibuhitensis reared in natural seawater had significantly greater body length,body weight,head length,head width,muscle weight,and body circumference(P<0.05).In addition,except the expression of IDH gene of female shrimps,the gene expression of PK,HK,IDH,and CPT1 were generally depressed in the artificial seawater groups(P<0.05),which may partially explain the hampered growth performance detected.