Abstract: By detecting nutritional components of E.fuscoguttatus ♀×E.lanceolatus ♂,E.lanceolatus,E.fuscoguttatus and E.awoara,the nutritional value of grouper was evaluated comprehensively.The protein,water,amino acids,minerals and fatty acids of four grouper species were detected by national standard biochemical assay.The protein content and crude fat content of four grouper species were 15.18%~17.33% and 0.81%~7.04%,respectively.18 kinds of amino acids were detected,while the essential amino acid was 41.43%~42.47% of total amino acids,which was higher than the FAO/WHO 40% scoring standard.The flavour amino acid and EAAI value were 6.34%~7.08% and 0.93~1.13,respectively,which indicated that the protein quality of four grouper species was excellent.Among the mineral elements of the four grouper species,K content was the highest of constant element and Fe content was the highest of trace element.In terms of fatty acid composition and content,22 amino acids were higher in E.lanceolatus than in the other three grouper species.The content of polyunsaturated fatty acid accounted for 38.14% of the total fatty acids.The four grouper species contain higher DHA+EPA content,accounting for 13.58%~27.57% of the total fatty acids.Therefore,the protein quality of the four kinds of grouper was excellent,the composition ratio of amino acid and fatty acid was appropriate,and the mineral content was rich.It was an excellent aquatic product with high nutritional value and high comprehensive utilization value.