氨海水和5-羟色胺对菲律宾蛤仔解剖配子体外促熟的影响

Effect of ammonia seawater and 5-HT on the maturation of gametes stripped from Ruditapes philippinarum

摘要: 采用解剖配子体外浸泡的方法,研究了氨海水和5-羟色胺(5-HT)对菲律宾蛤仔(Ruditapes philippinarum)配子的促熟作用。结果表明,采用一定浓度的氨海水和5-HT浸泡能促进菲律宾蛤仔卵子的生发泡破裂(Germinal vesicle breakdown,GVBD)(P<0.05),氨海水处理组的受精率优于5-HT处理组。其中,10 μmol/L 5-HT浸泡卵母细胞60 min受精率最高,达到28.38%;0.015%氨海水浸泡卵母细胞60 min受精率最高,达到41.28%。

Abstract: To construct the artificial breeding method of R.philippinarum, effects of ammonia seawater and 5-hydroxytryptamine (5-HT) on the maturation of gametes were studied by soaking the dissected gametes in vitro. Results showed that immersion with ammonia seawater and 5-HT significantly stimulated the germinal vesicles breakdown in R.philippinarum(P<0.05). However, the fertilization rate of ammonia seawater treatment group was better than that of 5-HT treatment group. The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 28.38%. The fertilization rate of oocytes treated with 0.015% ammonia seawater for 60 min was the highest (about 41.28%).