Abstract: Enterocytozoon hepatopenaei (EHP) is a specialized intracellular parasite that has caused huge economic losses in shrimp aquaculture due to its large outbreaks,and no specific drugs have been found to effectively treat EHP infection.Histopathology and molecular biology are the main methods used to detect EHP.The isothermal amplification method,a key technique in molecular biology,has gained significant attention as a potential field detection method owing to its simplicity,cost-effectiveness,high sensitivity,and specificity.This paper provides a comprehensive review of conventional detection methods for EHP,including loop-mediated isothermal amplification (LAMP) and recombinase isothermal amplification (RPA),and discusses their applications in EHP detection.The aim is to offer valuable insights for the early detection,efficient prevention,and control of EHP infection,ultimately promoting the sustainable development of shrimp aquaculture.