Abstract: The testing conditions were optimized base on the standard of determinating eight sulfonamides in aquatic products by post-column derivation high performance liquid chromatography (HPLC) method. In the optimized conditions, eight sulfonamides (sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxazole, sulfamethoxypyridazine, sulfisoxazole, sulfaquinoxaline) residues in aquatic products were separated and detected. The calibration curves were linear in a concentrations range of 0.025~0.5 μg/mL for sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine; 0.05~1.0 μg/mL for sulfamethoxazole, sulfamethoxypyridazine, sulfisoxazole and 0.1~2.0 μg/mL for sulfaquinoxaline, with r>0.9990.The average recovery was 84.2%~109% and the relative standard deviation was in the range of 1.64%~10.6% for spiked weever sample with 5 μg/kg~100 μg/kg of sulfonamides.