海带多糖和甘露醇的提取工艺研究
Research on extraction technology of polysaccharides and mannitols from Laminaria japonica
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摘要: 以海带作为原料,通过单因素试验和正交试验,研究了盐酸和柠檬酸质量分数、提取温度、提取时间、固液比对海带汁中多糖和甘露醇提取率的影响。试验结果最适提取条件为:提取液为1.0%盐酸、提取温度为100℃、固液比为1∶30、提取时间4 min。在此条件下对海带多糖和甘露醇进行提取得到多糖质量浓度为3.464 mg/g、甘露醇为152.963 mg/g。Abstract: In this experiment , Laminaria japonica as raw material was used to study the factors affecting the leach rate of polysaccharides and mannitol, including hydrochloric acid and citric acid content,temperature,time,solid-liquid ratio. Single factor and orthogonal experiments were done to determine the best leach conditions which was found as 1.0%HCl,100℃,4 min and at solid-liquid ratio of 1:30 . Under this condition we obtained 3.464 mg/g polysaccharides and 152.963 mg/g mannitol.
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