Abstract: In the paper, the morphological characteristics and fecundity, hatchability on different breeding groups of Litopenaeus vannamei broodstock were studied, respectively.The results showed that there were no significant difference between A group and B group except body weight, body length, carapace length, the second segment height and telson length.The spawning amount of A and B group were respectively (0.65±0.20)×10⁴, (0.64±0.18)×10⁴individual/body weight (g) (P>0.05).However, there were significant differences on the nauplii amount and hatching rate (P<0.05).There was significantly correlated on body weight and fecundity of B group of Litopenaeus vannamei broodstock (P<0.05), the broodstock spawning quantity (Y, million) and weight (W, g) regression equation was:Y=0.0545W^0.6778 (R²=0.5228).There was no significantly correlated on morphometric character and fecundity of A group of Litopenaeus vannamei broodstock (P>0.05), the broodstock spawning quantity (Y, million) and weight (W, g) regression equation was:Y=0.0103W^1.0878 (R²=0.4103).