Abstract: In the southeast coastal area of China,tetrodotoxin poisoning often occurred for eating puffer fish.Currently main detection methods of tetrodotoxin are mouse assay and enzyme linked immunosorbent assay(ELISA) etc.The tetrodotoxin detection methods presently need much more effort in time and cost,and can not meet the market requirements nowadays.We developed a tetrodotoxin colloidal gold immunochromatographic test kit,which the lowest detection limit was 100 ng/mL.The logic four parameter curve fitting,variation coefficient analysis,and standard test methods were used to test the stability,sensitivity,the fitting degree of the trend line,and detection range of homemade detection card in tetrodotoxin quantitative detecting.The results showed that the variation coefficient of homemade tetrodotoxin fluorescence immuno-chromatographic testing card was 0.124;correlation coefficient r² value was 0.995 5;the detection range was 2~600 ng/mL.The testing card can meet the need of tetrodotoxin detection.