Abstract: To provide technical support for artificial breeding of Pseudogobio vaillanti,the article explored the effect of starvation stress on the initial feeding and growth,and determined the point of no return(PNR)of early larvae.The changes of absorption of yolk sac,morphology,behavior,growth,survival and initial feeding rate of starved larvae were observed by methods of experimental ecology,and the PNR and optimized initial feeding time were determined.The results showed that under the water temperature of(25.5±1.2)℃,the larvae was started feeding at 4-day-old and the yolk sac was completely absorbed at 5-day-old.A period of mixed nutrition was only maintained 1 day.The initial feeding rate reached 100% at 5-6 days of age.The PNR of starved larvae occurred during 8-9 days of age with the interval between the first feeding to PNR was 4-5 days as the same time of 50% cumulative mortality rate for larvae of P.vaillanti.The total length had negative growth at 9 days of age.At 6 days of age,the body weight of starved group had negative growth and relative fatness decreased continuously,and it was significantly lower than that of control group,which could be used as judgment basis for the starved larvae of P.vaillanti.The results showed that the larvae of P.vaillanti had short mixed nutrition period and weak starvation tolerance.At water temperature(25.5±1.2)℃,the artificially bred larvae of P.vaillanti could be fed at 4-day-old.The best feeding time as 5-day-old,but the latest feeding time should not exceed 7-day-old.