Abstract: In this study, nutritional components in the body and muscle of a new hybrid grouper(E.fuscoguttatus ♀×E.polyphekadion ♂)were tested and analyzed with routine methods, in order to evaluate its nutritive value and offer basic date and theoretical basis for the research of its nutrition and the development of formula feed.The results showed that the ratio of flesh content was 74.31%, the contents of moisture, crude protein, crude fat, crude ash, Ca, and P of fresh body were 71.50%, 20.45%, 2.45%, 2.88%, 1.68% and 0.92%, respectively.The contents of moisture, crude protein, crude fat and crude ash of the hybrids’ fresh muscles were 74.60%, 20.52%, 2.48%and 1.23%, respectively.There were 18 kinds of amino acids in the muscle, including 8 essential amino acids (EAA) for human body and 4 delicious amino acids.The content of EAA was 6.89%, which accounted for 41.67% of the total amino acids.The content of tasty amino acids was 6.31%, which accounted for 38.60% of the total amino acids.The essential amino acid index (EAAI) was 78.21.The composition of EAA conforms to FAO/WHO standards.The proportion of saturated fatty acids (SFA) was 35.56%, and the proportion of polyunsaturated fatty acids (PUFA) was 36.46%.EPA and DHA accounted for 6.55% and 12.53% of the total fatty acids, respectively.10 kinds of mineral elements were detected in the muscle.The content of macro elements in muscle from high to low was K>P>Mg>Na>Ca.Among the trace elements, the content of Fe was the highest and the content of Se was the lowest.To sum up, the hybrid grouper(E.fuscoguttatus ♀×E.polyphekadion ♂) was a kind of high quality economic marine fish with good edible value and nutritive value, worthy of exploitation and utilization.