Optimization of preparation of chondroitin sulfate by alkali leaching method

The alkali leaching method was used in the extraction process of chondroitin sulfate from squid cartilage.Choosing chondroitin sulfate yield as evaluation index， the best technology parameters were confirmed by single factors and surface response optimization tests. The optimum conditions of leaching technology were obtained as follows ： ratio of material to solvent was 1: 8 ( g /m L ) ，leaching temperature was 5 0 C ，leaching time was 5. 3 h ，alkali concentration was 3 % . Under these conditions，the yield of chondroitin sulfate from squid cartilage was 62. 89% .