罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析

蒋飞; 戴习林

doi:10.14012/j.cnki.fjsc.2023.01.002

罗氏沼虾3个不同群体线粒体COⅠ基因序列变异及遗传多样性分析

蒋飞¹,
戴习林^2, ,

1. 上海市水产研究所, 上海市水产技术推广站, 上海 200433;
2. 上海海洋大学, 水产科学国家级实验教学示范中心, 农业部淡水水产种质资源重点实验室, 水产动物遗传育种中心上海市协同创新中心, 上海 201306

基金项目:

上海市虾类产业技术体系建设项目(沪农科产〔2014〕第5号)

详细信息

作者简介:
蒋飞(1989-),男,工程师,硕士,研究方向:水产养殖及育种研究。E-mail:ygljf214@163.com

通讯作者:
戴习林(1969-),男,教授,研究方向:水产养殖及育种研究。E-mail:xldai@shou.edu.cn

中图分类号: S917
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出版历程
- 收稿日期: 2022-06-19
- 刊出日期: 2023-02-24

Analysis of the genetic diversity and sequence variation of mitochondrial COⅠgene from three populations of Macrobrachium rosenbergii

JIANG Fei¹,
DAI Xilin^2, ,

1. Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, China;
2. National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Collaborative Innovation of Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China

摘要

摘要: 为研究上海选育群体(SC)、浙江养殖群体(ZJ)和泰国野生群体(TL)3个罗氏沼虾群体的遗传多样性,实验对3个不同群体的线粒体CO Ⅰ 基因序列进行PCR扩增和测定。在长度为458 bp的CO Ⅰ 基因序列中,只检测到8个变异位点和4个单倍型;平均A+T含量(54.4%)高于G+C含量(45.7%)。遗传变异参数分析显示,单倍型多样性指数最高为TL群体(Hd=0.733),核苷酸多样性指数最高为ZJ群体(π=0.005)。AMOVA分析表明,来自群体间的遗传变异(74.5%)高于来自群体内的遗传变异(25.5%)。采用NJ法和UPMGA法构建的系统进化树的趋势一致,都显示SC群体和ZJ群体遗传关系最近,首先聚在一起成为一小支,然后再与TL群体聚为另一支。研究结果可为罗氏沼虾种质资源保护和利用提供参考。
- 罗氏沼虾 /
- COⅠ基因 /
- 遗传多样性 /
- 遗传分化
Abstract: In order to investigate the genetic diversity among three populations of Macrobrachium rosenbergii collected from Shanghai breeding population (SC), Zhejiang cultured population (ZJ) and Thailand wild population (TL), the mitochondrial sequences of the COⅠ gene in the three populations were amplified and determined by PCR and sequencing. The result showed that 8 nucleotide sites and 4 haplotypes were found in the 458 bp long partial gene segment. The mean content of A+T (54.4%) was higher than G+C (45.7%). Genetic variation parameters showed the population of highest haplotype diversity was TL population (Hd=0.733), the population of highest nucleotide diversity was ZJ population (π=0.005). AMOVA showed that genetic variations among populations (74.5%) was higher than that within populations (25.5%). The tendency of molecular phylogenetic trees which were constructed by NJ and UPMGA method was similar. SC population and ZJ population were the closest genetically, and they clustered into a small branch, then with TL population. These findings were expected to provide reference information for the protection and utilization of germplasm resources of M.rosenbergii.
- Macrobrachium rosenbergii /
- COⅠ gene /
- genetic diversity /
- genetic differentiation

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参考文献(24)

[1]	杨明,丁福江.池养罗氏沼虾性二型的研究[J].渔业研究,2020,42(4):372-379.
[2]	张天澍,王玉凤.罗氏沼虾的染色体研究[J].华中师范大学学报(自然科学版),2003,37(2):231-232.
[3]	张海琪,何中央,徐晓林,等.罗氏沼虾肌肉 8种同工酶的研究[J].海洋湖沼通报,2004(1):32-37.
[4]	蒋飞,戴习林,朱其建,等.罗氏沼虾5个专门化品系选择系生长比较及其遗传结构分析[J].上海海洋大学学报,2014,23(3):329-337.
[5]	Mohanty P,Sahoo L,Parida K,et al.Development of polymorphic EST-SSR markers in Macrobrachium rosenbergii by data mining[J].Conservation Genetics Resources,2013,5:133–136.
[6]	高俊娜,刘萍,李健,等.日本蟳线粒体16S rRNA和COⅠ基因序列比较分析[J].中国海洋大学学报,2010,40(12):57-63.
[7]	Brown W M,George M Jr,Wilson A C.Rapid evolution of animal mitochondrial DNA[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76(4):1967-1971.
[8]	王鹏云.双线紫蛤COⅠ基因序列多态性与水环境分析[J].渔业研究,2020,42(4):324-338.
[9]	Moore D D,Strauss W M.Preparation of genomic DNA from mammalian tissue[J].Current Protocols in Molecular Biology,1998:221-223.
[10]	Abinash P.Geographic variation within a tandemly repeated mitochondrial DNA D-loop region of a North American freshwater fish,Pylodictis olivaris[J].Gene,2014,538(1):63-68.
[11]	Hallerman E M.Population genetics:principle and applications for fisheries scientist[J].American Fisheries Society,2003,5:59-100.
[12]	Xu H,Zhang Y,Xu D,et al.Genetic population structure of miiuy croaker (Miichthys miiuy) in the Yellow and East China Seas base on mitochondrial COⅠ sequences[J].Biochemical Systematics and Ecology,2014,54:240-246.
[13]	李咏梅,陈秀荔,赵永贞,等.钦州湾牡蛎线粒体16S rRNA和CO Ⅰ 基因片段的序列变异分析[J].广东海洋大学学报,2009,29(3):11-18.
[14]	Carini G,Hughes J M.Population structure of Macrobrachium australiense (Decapoda:Palaemonidae)in Western Queensland,Australia:the role of contemporary and historical processes[J].Heredity,2004,93:350-363.
[15]	Vassilios K,Apostolos A,Alexandrost,et al.Development of mitochondrial DNA primers for use with Homarid Lobsters[J].Marine Biotechnology,2003,5:469-479.
[16]	姜虎成,冯建彬,丁怀宇,等.淮河水系日本沼虾群体遗传结构和系统演化的线粒体COⅠ序列分析[J].动物学杂志,2012,47 (2):73-84.
[17]	葛家春,许志强,李晓晖,等.利用线粒体COⅠ序列分析4水系中华绒螯蟹群体遗传学特征[J].中国水产科学,2011,18 (1):16-22.
[18]	潘贤辉,周康奇,陈忠,等.基于线粒体D-loop区和COⅠ基因序列研究2个禾花鲤群体和野生鲤群体的遗传多样性与系统进化关系[J].淡水渔业,2019,49(6):33-41.
[19]	You E M,Chiut S,Liu K F,et al.Microsatellite and mitochondrial haplotype diversity reveals population differentiation in the tiger shrimp (Penaeus monodon) in the Indo-Pacific region[J].Animal genetics,2008,39(3):267-277.
[20]	Leberg P L.Effects of population bottlenecks on genetic diversity as measured by allozyme electrophoresis[J].Evolution,1992:477-494.
[21]	姚茜,杨频,陈立侨,等.罗氏沼虾三群体线粒体D-loop基因序列差异的初步研究[J].水产学报,2007,31:18-22.
[22]	于爱清,李思发,蔡完其."新吉富" 罗非鱼选育 F(10)~F(13) 遗传变异微卫星分析[J].上海海洋大学学报,2011,20(1):1-7.
[23]	Balloux F,Lugon-Moulin N.The estimation of population differentiation with microsatellite markers[J].Molecular ecology,2002,11(2):155-165.
[24]	Hebert P D N,Cywinska A,Ball S L,et al.Biological identification through DNA barcodes[J].Proceedings of the Royal Society B Biological Sciences,2003,270(1512):313-321.

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