Analysis of antioxidant activity of polysaccharide from sea cucumber and fermented sea cucumber by Bacillus natto
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摘要: 为了分析纳豆菌发酵海参制备的多糖的抗氧化活性,研究采用分光光度法测定发酵海参制备的多糖和海参多糖的羟自由基清除能力、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除能力和铁还原能力。结果表明,发酵海参制备的多糖的羟自由基清除能力和DPPH自由基清除能力低于海参多糖,ABTS自由基清除能力高于海参多糖,两种多糖均没有铁还原能力。本研究为开发新型发酵海参精深加工产品提供了理论依据。Abstract: In order to analyze the antioxidant activity of polysaccharide from sea cucumber and fermented sea cucumber by Bacillus natto, the hydroxyl radical scavenging ability, DPPH radical scavenging ability, ABTS radical scavenging ability and iron reducing ability were detected by spectrophotometry.The results showed that the hydroxyl radical scavenging ability and DPPH radical scavenging ability of polysaccharides from fermented sea cucumber were lower than those of polysaccharides from sea cucumber, but ABTS radical scavenging ability was higher than that of polysaccharides from sea cucumber, and both polysaccharides had no iron reducing ability.This study provided a scientific basis for the development and application of new type sea cucumber products.
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Keywords:
- sea cucumber polysaccharides /
- antioxidant activity /
- Bacillus natto
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