Study on the mitochondrial genome and phylogeny of <i>Rhinogobius changtinensis</i>

ZENG Sheng; CHEN Xiaohong; ZHONG Shen; YANG Deyuan; MENG Yuanzheng; LIN Zeyang; WEI Shujian; ZHU Yuchen; XU Yibin

doi:10.14012/j.jfr.2024164

Journal of Fisheries Research > > Corrected proof > DOI: 10.14012/j.jfr.2024164

Zeng S，Chen X H，Zhong S，et al. Study on the mitochondrial genome and phylogeny of Rhinogobius changtinensis[J]. Journal of Fisheries Research，xxxx，xx（x）：1 − 13. DOI: 10.14012/j.jfr.2024164

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Study on the mitochondrial genome and phylogeny of Rhinogobius changtinensis

1.
College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
2.
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
3.
Xiamen Ocean Vocational College, Xiamen 361102, China
4.
Xiamen Ocean Center, Ministry of Natural Resources, Xiamen 361101, China
5.
City Universities of Macau, Macau 999078, China

More Information

Received Date: December 24, 2024
Revised Date: February 18, 2025
Accepted Date: February 28, 2025

Graphical Abstract

Abstract

Abstract

Objective
The genus Rhinogobius is highly diverse in species, and exhibits significant variation in morphological traits, makeing traditional morphological methods unreliable for accurate species identification and classification. Rhinogobius changtinensis, a species in this genus, lacks clear mitochondrial genomic data, and its phylogenetic relationships remain poorly understood. This study aims to analyze the mitochondrial genome of R. changtinensis and provide insights into species identification, classification, and the phylogenetic study of the genus Rhinogobius.
Methods
Using the Illumina NovaSeq X Plus platform, the study conducted next-generation sequencing on the mitochondrial genome of R. changtinensis, followed by the assembly and annotation of the genome sequence, and then analyzed the structural features and base composition of the mitochondrial genome. Based on the nucleotide sequences of 13 protein-coding genes, the study constructed a phylogenetic tree using maximum likelihood (ML) and Bayesian inference (BI) methods.
Results
The mitochondrial genome of R. changtinensis was 16 487 bp in length, comprising 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and an A+T-rich region of 854 bp. The gene arrangement was consistent with most species within the genus. Phylogenetic analysis indicated that R. changtinensis was the most closely related to Rhinogobius duospilus (PP=1, BS=100%).
Significance
This study provides valuable mitochondrial genomic data for R. changtinensis and offers crucial insights into the phylogenetic relationships within the genus Rhinogobius, contributing to the understanding of its systematics.
- Rhinogobius,
- mitochondrial genome,
- gene structure,
- phylogeny

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References (39)

References

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Study on the mitochondrial genome and phylogeny of Rhinogobius changtinensis

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