Research on extraction technology of polysaccharides and mannitols from Laminaria japonica

In this experiment , Laminaria japonica as raw material was used to study the factors affecting the leach rate of polysaccharides and mannitol, including hydrochloric acid and citric acid content,temperature,time,solid-liquid ratio. Single factor and orthogonal experiments were done to determine the best leach conditions which was found as 1.0%HCl,100℃,4 min and at solid-liquid ratio of 1:30 . Under this condition we obtained 3.464 mg/g polysaccharides and 152.963 mg/g mannitol.